Human interleukin-1? induces A375-S2 human melanoma apoptosis through caspase pathway
- VernacularTitle:人白细胞介素1?通过caspase途径诱导人黑色素瘤A375-S2细胞凋亡
- Author:
Che WANG
;
Minwei WANG
;
Shinichi TASHIRO
;
Satoshi ONODERA
;
Takashi IKEJIMA
- Publication Type:Journal Article
- Keywords:
Interleukin-1;
Melanoma;
A375-S2 cells;
Apoptosis;
Caspases
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the molecular biological mechanism and signal transduction pathway of interleukin-1? (IL-1?)-induced apoptosis in A375-S2 melanoma cells. METHODS: Photomicrocropy showed typical apoptotic changes. The cytotoxic effect of IL-1? in vitro and influences of caspases in this effect were measured by MTT assay. The cytotoxicity of cells was assessed by LDH-based assay. Degradation of DNA was detected by agarose gel electrophoresis. RESULTS: The inhibitory effect of IL-1? on A375-S2 cell growth was in a dose and time-dependent manner, and cell death rate reached more than 90% at 72 h after treatment with 10~(-9)mol/L IL-1?. The inhibitors of caspase-family, -1, -3, -8, -9, and -10, partially blocked cell death at early stage. LDH assay showed that major IL-1?-induced cell death was apoptosis, and in a dose and time-dependent manner. Typical apoptotic DNA ladder was observed in agarose gel electrophoresis. CONCLUSION: IL-1? induced apoptosis in melanoma A375-S2 cells by activating caspase pathway. [
