Effect of salidroside on mitochondrial membrane potential during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells
- VernacularTitle:红景天甙对缺氧/缺糖损伤的SH-SY5Y细胞线粒体膜电位的影响
- Author:
Wensheng ZHANG
;
Lingqun ZHU
;
Ruichun DENG
;
Fuling NIU
;
Rong TIAN
- Publication Type:Journal Article
- Keywords:
Salidroside;
SH-SY5Y cells;
Mitochondria;
Membrane potentials;
Apoptosis;
Anoxia;
Hypoglycemia
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of salidroside on intracellular free calcium concentration (([Ca~(2+)]i)), apoptosis, mitochondrial membrane potential (MMP) and activity during injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cells. METHODS: Mitochondrial activity was measured by methylthiazolyl tetrazolium test. MMP, [Ca~(2+)]i and apoptosis were measured by flow cytometry. RESULTS: SH-SY5Y cells were cultured in a hypoxia/hypoglycemia condition for 2, 4, 6 and 12 h, [Ca~(2+)]i and apoptosis rate significantly increased compared with control group (P
