Effects of L-arginine on the hypothermic perfusion and on preservation of isolated pancreas in canines
- VernacularTitle:L-精氨酸在犬离体胰腺低温灌注与保存中的作用
- Author:
Chunhui YUAN
;
Guichen LI
;
Yongfeng LIU
;
Jiang LIANG
;
Sanguang HE
- Publication Type:Journal Article
- Keywords:
PANCREAS/physi opathol;
REPERFUSION INJURY;
ARGININE;
HYPOTRERMIA,INDUCED
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of L-arginine on the reperfusion injury of hypothermic perfusion and on preservation of isolated pancreas(ISP) in canines . Methods After segmental pancreatic transplantation(SPTS) model were set up,the animals were randomly divided into 3 groups:(1)ISP irregulated and preserved with Euro Colln Solution(ECS)plus L-arginine(L-arg group);(2)IPS irregulated and preserved with ECS plus N-nitro-L-arginine methy ester(L-NAME group);and(3)IPS irregulated and preserved with ECS plus normal saline(Control group).The IPS were irregulated and preserved at 0-4℃ for 24h.Serum concentration of amylase and lipase were detected. NO,NOS and myeloperoxidase (MPO) and expression of iNOS mRNA in the pancreas graft were measured. Histological examination was performed. Results The level of NO in the L-arg group was significantly higher than that in the control group(P
