Preliminary Isolation and Purification of Spermatogonial Stem Cells
- VernacularTitle:小鼠生精干细胞的初步分离与纯化
- Author:
Quanan JIA
;
Ci ZHANG
;
Linglong WANG
- Publication Type:Journal Article
- Keywords:
Spermatogonial stem cells;
Isolation;
Purificaiton
- From:
Journal of Chinese Physician
2002;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain a simple method to isolate and purify spermatogonia stem cells. Methods The testis of newborn mouse were digested by modified two step digest method, and the type A spermatogonia cells were separated by different adhesive time with Sertoli cells. Results High concentration (95%) purified type A spermatogonia cells were acquired, and the spermatogonia transplanting experiment demonstrated the efficiency of the method.Conclusions This method is easy and efficient to isolation and purification of spermatogonial stem cells.
