Changes of myocardial nuclear membrane Ca~(2+)-ATPase function in ischemia/reperfusion injury
- VernacularTitle:心肌缺血再灌注损伤时细胞核膜钙泵功能的改变
- Author:
Ailing FU
;
Huamei HE
;
Lezhi ZHANG
- Publication Type:Journal Article
- Keywords:
Myocardial reperfusion injury;
Ca(2+)-transporting ATPase;
Cell nucleus
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The changes of myocardial nuclear membrane Ca 2+ -ATPase function was investigated in ischemia/reperfusion injury. METHODS: The model of myocardial ischemia/reperfusion injury was established in rats. Myocardial nuclei were purified with sucrose density centrifugation,the activity of Ca 2+ -ATPase was measured and calcium uptake was assayed with [ 45 Ca 2+ ] . RESULTS: Plasma levels of malondialdehyde (MDA) and free fatty acid (FFA) in myocardial ischemia/reperfusion injury increased significantly( P
