Signal peptide sequence of human interleukin-2 influenced hEndostatin gene expression and protein secretion in HepG2 cells
- VernacularTitle:hIL-2分泌肽序列在HepG2细胞中对hEndostatin表达和分泌差异的影响
- Author:
Tao YUE
;
Peng LIU
;
Qingli DENG
;
Ping ZHANG
;
Qiongmei JI
;
Haitao ZHANG
;
Zheny ZHU
- Publication Type:Journal Article
- Keywords:
Endostatin;
Neoplasm angiogenesis;
Signal peptide;
Interleukin-2
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: The role of human interleukin-2(IL-2) signal peptide sequence in the effect of human Endostatin (hEndostatin) expression and secretion was investigated in HeG2 cells. METHODS: RT-PCR and Western-blotting were conduct to observe mRNA level difference of hEndostatin gene, its protein expression and secretion level difference between with hIL-2 signal peptide sequence and without it. RESULTS: mRNA level of hEndostatin gene in HepG2 (pBlast-hIL2-hEndo) cells was higher than that in HepG2(pBlast-hEndo)( P
