Transferrin-bound Yb_2 uptake by U-87 MG cells and effect of Yb on proliferation of the cells
- VernacularTitle:转铁蛋白结合镱跨膜转运及镱对U-87 MG细胞增殖的影响
- Author:
Kui WANG
;
Xiulian DU
;
Yanzhong CHANG
;
Rongchang LI
;
Hongzhe SUN
;
Zhongmin QIAN
- Publication Type:Journal Article
- Keywords:
Yb 3+ / Yb 2Tf;
Transferrin/transferrin receptor;
Glioma;
U-87 MG cells
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the role of transferrin/transferrin receptor system in transferrin-bound Yb 2 (Yb 2Tf) uptake by U-87 MG cells and the effect of transferrin-bound and -free Yb 2 on proliferation of U-87 MG cells. METHODS: Cell culture and ICP-MS measurement of Yb 2. RESULTS: Yb 2Tf uptake by U-87 MG cells increased with the concentrations of Yb 2Tf, and reached saturation as the concentration in the incubation medium was raised to about 2 ?mol/L. Also, Yb 2 uptake by the cells increased with increase of the mole ratio (Yb 2: apoTf), reaching a maximum at 1.5 mole ratio. Yb 2Tf in 0.4 ?mol/L significantly inhibited proliferation of U-87 MG cells, however, 10 ?mol/L Yb 3+ had no significant effect on proliferation of the cells. CONCLUSION: The uptake of Yb 2 by U-87 MG cells might be mediated by transferrin/transferrin receptor system. Transferrin-bound but not transferrin-free Yb 2 could significantly inhibit proliferation of U-87 MG cells.
