Preparation and identification of anti-angiostatin monoclonal antibodies
- VernacularTitle:鼠抗人血管抑素单克隆抗体的制备和鉴定
- Author:
Cui ZHANG
;
Wenju LU
;
Yuzhao HE
;
Liyong HU
;
Qian FU
- Publication Type:Journal Article
- Keywords:
Angiostatin;
Antibodies,monoclonal;
Rats
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To further study the anti-tumor effect of angiostatin, an anti-human angiostatin monoclonal antibody was prepared and identified.METHODS:The hybrodoma techniques were used. The BALB/C mice were immunized with angiostatin. The supernatant of cell culture were collected and screened by ELISA and double immunodiffusion.RESULTS: There cell lines which steadily secreted the anti-angiostatin monoclonal antibody were identified by ELISA and double immunodiffusion. The antibody was IgG1 and specifically recognized angiostatin without crossing reactions to rhIL-2, rhTNF-?, rhIFN-? and serum proteins.CONCLUSION: The antibodies secreted by three hybridoma cell lines identified by several methods were specific antibodies of angiostatin.
