Telomerase Activity in Condyloma Acuminatum:Correlation with Decreased Apoptosis
- VernacularTitle:端粒酶活性与细胞凋亡在尖锐湿疣患者组织中的表达
- Author:
Hongfu XIE
;
Hao FENG
;
Xuegong FAN
;
Wei SHI
;
Mingliang CHEN
;
Qianjun DU
;
Xiang CHEN
- Publication Type:Journal Article
- Keywords:
Condylomata acuminata;
Telomerase;
Apoptosis
- From:
Chinese Journal of Dermatology
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the telomerase activity,the keratinocyte apoptosis in condyloma ac-cuminatum(CA)and the correlation between them.Methods CA specimens from30patients were stud-ied,and compared with normal tissue and tumor cell lines.Telomerase activity was detected with telomeric repeat amplification polymerase chain reaction(TRAP)-ELISA.Terminal deoxynucleotidyl transferase(TdT)-mediated biotin dUTP nick end-labeling(TUNEL)was used to evaluate apoptotic cells.Results Increased telomerase activity was detected in27(90%)patients with CA,with A 450 ranging between0.50and2.76(mean1.3022).Apoptotic keratinocytes were found in24out of30CA cases(80%).A statistically signifi-cant inverse correlation was found between telomerase activity and apoptotic index(r =-0.52,P=0.004).Conclusion Keratinocyte telomerase is activated in condyloma acuminata,which is correlated with the downregulation of apoptosis,thus they might be involved in the pathogenesis of CA.
