Biological characteristics of tumor apoptotic cells-plused-dendritic cells induced by tumor lysates supernatants
- VernacularTitle:肿瘤细胞冻融裂解物上清对凋亡细胞负载的树突状细胞生物学特性的作用研究
- Author:
Tao GU
;
Yibei ZHU
;
Min LI
;
Yong HUANG
;
Hong XI
;
Gehua YU
;
Jun DAI
;
Zongjiang GU
;
Xueguang ZHANG
- Publication Type:Journal Article
- Keywords:
Dendritic cells;
Tumor lysate supernatants;
Apoptosis;
T-lymphocytes, cytotoxic
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To prepare the efficient tumor-DC vaccines, dendritic cells(DC) derived from 6-8 weeks Balb/c mice bone marrow progenitor cells were pulsed by apoptotic SP2/0 tumor cells and induced maturation by SP2/0 tumor lysates supernatants. Then SP2/0 tumor burdening Balb/c mice were immunized by the tumor-DC vaccines to observe the therapeutic effects in vivo .METHODS: Immature DC were derived by recombinant murine GM-CSF and IL-4, then were pulsed by SP2/0 apoptotic cells. Tumor-DC vaccines were stimulated by LPS and SP2/0 tumor lysates supernatants prepared by four cycles repetitive freezing and thawing, respectively. -thymidine incorporation test and standard 4h [ 51 Cr] release assay were used to detect the proliferation and activation of cytotoxic T lymphocytes (CTL) stimulated by DC in vitro . (4-5)?10 5 DC were immunized in the right inguen of SP2/0 tumor burdening Balb/c mice and most mice received three cycles immunization every two weeks. Changes of the tumor and mice life-spans were recorded. RESULTS: In vitro proliferation and activation of CTL induced by the tumor-DC vaccines of tumor lysates supernatants or LPS stimulation group were more powerful than other groups ( P
