Proliferation and apoptosis of human umbilical vein endothelial cells induced by oxidized low density lipoprotein
- VernacularTitle:氧化低密度脂蛋白对人血管内皮细胞ECV-304促增殖及诱导凋亡的作用
- Author:
Ying QIN
;
Jun YANG
;
Lingqun ZHU
;
Fuling NIU
;
Wei CUI
;
Shuoren WANG
;
Liangdu JIANG
- Publication Type:Journal Article
- Keywords:
Lipoproteins, LDL;
Endothelial cell;
Apoptosis;
Atherosclerosis
- From:
Chinese Journal of Pathophysiology
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate proliferation and apoptosis of cultured endothelial cell (ECV-304 cell line) induced by varied concentrations of oxidized low density lipoprotein (ox-LDL). METHODS: Cell morphology, Typan blue test, MTT test, LDH release test, flow cytometry and micro-molecular weight DNA fragment gel electrophoresis of apoptosis were used for the detection of the cytotoxic effects of ox-LDL on ECV-304 cell line. RESULTS: 0 1, 1, 10 mg/L ox-LDL could promote proliferation of ECV-304 cells. When the concentration of ox-LDL reached up to 100 mg/L and above, the distinct cytotoxic effect appeared. Further study showed that the apoptosis rate of endothelial cells, induced by ox-LDL of 150 mg/L and 200 mg/L for 12 hours, are 15 86% and 21 89%, respectively. 18 h and above hours after incubation, the apoptosis rate began to decrease and rate of necrosis increased. CONCLUSION: ox-LDL has strong cytotoxic effects on endothelial cells and could give rise to different pathologic process, such as proliferation, apoptosis prophase, apoptosis and necrosis.
