Study on differentially expressed genes in ischemic myocardium and the effect of Sini decoction on them by DNA microarray
- VernacularTitle:应用基因表达谱芯片观察小鼠心肌缺血后基因表达的变化以及四逆汤对其影响
- Author:
Weikang WU
;
Hongmei TAN
;
Hanchuan LUO
;
Mingqi ZHAO
;
Tianwen LIANG
- Publication Type:Journal Article
- Keywords:
Oligonucleotide array sequence analysis;
Gene expression;
Myocardial ischemia;
Sini decoction
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To screen the differentially expressed genes among normal, ischemic and Sini decoction-treated myocardium using DNA microarray.METHODS: Kunming mice were randomly divided into control group, ischemic group and Sini decoction group. Total RNA was extracted from myocardium of each group. cDNA microarray chips containing 2 304 cDNAs were used to investigate the gene expression pattern of each group. RESULTS: Up-and down-regulated genes were 33 and 70 in ischemic group vs control group, respectively. Up-and down-regulated genes were 23 and 52 respectively in Sini decoction group vs ischemic group. CONCLUSIONS: The analysis of gene expression pattern of ischemic myocardium based on cDNA microarray can realize high-throughput screening of the genes. Further analysis of those obtained genes information will be helpful to understand the molecular mechanism of myocardial ischemia and the therapeutic mechanism of Sini decoction.
