Human mesenchymal stem cells differentiate into neuron-like cells by increase in intracellular cyclic AMP
- VernacularTitle:胞内cAMP浓度的增加可诱导骨髓间质干细胞分化为神经元样细胞
- Author:
Peng XIANG
;
Wenjie XIA
;
Lirong ZHANG
;
Zhenguang CHEN
;
Xiuming ZHANG
;
Yan LI
;
Shunon LI
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Stem cells;
Neurons;
Adenosine cyclic monophosphate;
Forskolin
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the differentiation from human mesenchymal stem cells(hMSC) into neuron-like cells by the increase in intracellular cyclic AMP. METHODS: hMSC were separated from human marrow with Ficoll-Paque reagent and expanded in culture medium. hMSC were induced to differentiate into neurons with Forskolin and 3-isobutyl-1-methyl-xanthine (IBMX). Neuron-specific enolase(NSE), neurofilament(NF), glial fibrillary acaidic protein(GFAP) were detected by immunohistochemistry. RESULTS: hMSC were expanded as undifferentiated cells in culture for more than 10 passages. Forskolin/IBMX can induce hMSC to exhibit a neuronal phenotype, expressing NSE and NF-M in 5 hours. But the neuron-like cells didn't express the glial astrocyte marker GFAP. CONCLUSION: hMSC can be induced to differentiate into neurons by increase in the intracellular cAMP.
