Detection of CD23 mRNA expression in B-cell lymphoma by in situ hybridization with digoxigenin-labelled DNA probe
- VernacularTitle:地高辛素标记探针原位杂交法检测B淋巴瘤CD23基因的表达
- Author:
Lin WANG
;
Bin WU
;
Chunxiao SU
;
Shunon LI
- Publication Type:Journal Article
- Keywords:
In situ hybridization;
Digoxigenin;
DNA probes;
Lymphoma, B-cell;
Receptors, IgE
- From:
Chinese Journal of Pathophysiology
1999;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To detect CD23 mRNA expression in B-cell lymphoma. METHODS: 20 pathological diagnostic conformed B-cell lymphoma samples were selected. Surface CD23 protein expression was examined by SP-immunohistochemistry and CD23 mRNA was detected by in situ hybridization with digotoxin labeled CD23 DNA probe.RESULTS: The positive rates of both surface CD23 protein and CD23 mRNA expression in B-cell lymphoma samples were more than 90%. CONCLUSIONS: The high expression of CD23 was showed in B-cell lymphoma both in mRNA and in protein levels. The results of this study was useful for understanding the molecular mechanism of B-cell lymphoma and for clinical diagnosis and treatment of the disease.
