Determination of Aristolochic Acid A in Decoction of Caulis Aristolochiae Manshuriensis by RP-HPLC
- VernacularTitle:反相高效液相色谱法测定关木通水煎剂中马兜铃酸A含量
- Author:
Lin WU
;
Weihui SU
;
Shizhen ZHOU
- Publication Type:Journal Article
- Keywords:
Caulis Aristolochiae Manshuriensis;
Aristolochic acid A;
RP-HPLC
- From:
China Pharmacy
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a RP-HPLC method for the determination of aristolochic acid A in decoction of Caulis Aristolochiae Manshuriensis METHODS:The analytical column was Spherisorb ODS2 column(4 6mm?250mm,5?m) The mobile phase consisted of a mixture,methanol-water-acetic acid(70∶27∶1) The flow rate was 1 0ml/min The UV detection wavelength was 250nm RESULTS:The linear range was 0 0 128?g～0 4 096?g(r=0 9 999) The regression equation was Y=4 553 7+5 388 319 3X The average recovery of aristolochic acid A was 97 33%(RSD=2 34%) CONCLUSION:This method is simple,sensitive and accurate
