Role of nitric oxide in the inhibition of lipopolysaccharide-induced thoracic aortic hyporeactivity by cholecystokinin in vitro
- VernacularTitle:一氧化氮在八肽胆囊收缩素抗脂多糖致离体兔胸主动脉低血管反应性中的作用
- Author:
Shujin LI
;
Yiling LING
;
Dianhua WANG
;
Zhenyong GU
;
Aihong MENG
;
Tienian ZHU
- Publication Type:Journal Article
- Keywords:
Cholecystokinin;
Lipopolysaccharides;
Aorta, thoracic;
Nitric oxide
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of cholecystokinin octapeptide(CCK-8) on the L-arginine-nitric oxide(NO) pathway in rabbit thoracic aortae treated with lipopolysaccharide(LPS). METHODS: The isolated thoracic aortic rings(TARs) from rabbits were incubated with LPS, LPS+CCK or vehicle for 14 h. Then the contractility to phenylephrine(PE) by TARs and the response to L-arginine(L-Arg) by pre-contractile TARs were measured. In addition, we added NO synthase(NOS) inhibitors aminoguanidine(AG)and N ?-nitro-L-arginine(L-NNA) into organ baths to observe the changes of vascular contractility to PE. NOS activity in isolated TARs were also detected. RESULTS: Incubation of TARs with LPS for 14 h resulted in an increase of NOS activity and a reduction of contractility to PE. Treatment with CCK-8 significantly inhibited the increased NOS activity in thoracic aortae and improved the hypocontractility of TARs to the same degree as AG. CONCLUSION: CCK-8 may improve the hypocontractility of TARs induced by LPS by inhibiting the activity of NOS.
