A novel cDNA clone related with rat liver regeneration
- VernacularTitle:一株与大鼠肝细胞再生相关的新cDNA克隆
- Author:
Siying WANG
;
Jie CHENG
;
Hong ZHENG
;
Ping ZHANG
;
Baoting ZHANG
;
Wangxiang XU
;
Handong WEI
;
Xiaomin YANG
- Publication Type:Journal Article
- Keywords:
Hepatocytes;
Genes;
Rats
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study a novel gene that probably related with liver regeneration, which was found by representational difference analysis(RDA). METHODS: cDNA sequence, tissue distribution and functions of the novel gene were studied by slot blot, Northern blot, RT-PCR, cDNA library screening and sequence analyzing. RESULTS: Two full-length clones were isolated from cDNA library of rat fetal livers and the sequence analysis identified that the positive cDNA encoded 76 amino acids only; Using the cDNA as a probe, the novel gene showed a specific liver distribution, a moment increasing expression in one hour after partial hepatectomy (PH) and high expression in fetal liver or liver tumor by Northern blot; EGF quickly induced its high expression in primary culture rat hepatocytes(FCS free).CONCLUSION: These results show that the novel gene is an early phase response gene that is closely related to a liver regeneration adjustment. It may encode peptide or has longer sequence at N tip.
