Serum immunopharmacological assessment:effects of cycloheximide on the activation of T lymphocytes of mouse
- VernacularTitle:放线菌酮对小鼠T细胞活化影响的血清免疫药理评价
- Author:
Shiming QUAN
;
Yaoying ZENG
;
Xianhui HE
;
Shan ZENG
;
Jingfang DI
;
Jingxian ZHAO
;
Yi LING
- Publication Type:Journal Article
- Keywords:
T-lymphocytes;
Cycloheximide;
Serum immunopharmacology;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of cycloheximide on the T cells activation by mitogen in vitro with CD69 expression as activation marker for the application of this drug clinically. METHODS:Lymphocytes were isolated from lymphoid nodes of C57BL/6 mouse. The cells were preincubated with cycloheximide(CHX), 5% serum containing CHX respectively for an hour, then further incubated with polyclonal activators (Con A or PDB). Harvesting the cells after whole incubation for 24 h, we estimated the expression rates of CD69 on T cells by flow cytometry following two-color immunofluorescent staining. RESULTS: The expression rates of CD69 on the T cells preincubated with CHX, serum containing CHX after the stimulation in response to Con A or PDB all showed significant difference with the expression rates of control group, respectively ( P
