Reestablishment of cell polarity of hepatocytes in vitro
- VernacularTitle:体外肝细胞极性的重建
- Author:
Xianjie ZHANG
;
Jiabang SUN
;
Haichen SUN
- Publication Type:Journal Article
- Keywords:
Cell polarity;
In vitro
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To reestablish cell polarity of hepatocytes cultured in vitro.Methods Sandwich configuration was used to culture hepatocytes. The morphology as well as specific plasma proteins were compared with a single collagen configuration.Results In sandwich configuration hepatocytes were arranged in single cell plate, and the gradual development of bile canaliculi like structures and an anastomotic network were observed over the course. Histochemical immunolocation revealed that membrane proteins were distributed specifically. Hepatocytes in single collagen configuration showed hardly any hepatic plate like structure,and the distribution of specific plasma protein was not formed. Conclusions Sandwich cultured hepatocytes reestablished cell polarity structurally and regained the characteristics of hepatocytes in situ.
