Effect of p19ARF on growth and apoptosis of leukemia cells
- VernacularTitle:p19ARF对白血病细胞增殖与凋亡的影响
- Author:
Xueyuan BAI
;
Fengxiang CHE
;
Yuxiang MA
;
Jinsong LI
;
Junhu ZHAI
- Publication Type:Journal Article
- Keywords:
Genes;
Cell cycle;
Apoptosis
- From:
Chinese Journal of Pathophysiology
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effect of p19ARF on the biological behavior of human leukemia cells. METHODS: p19ARF was cloned in eukaryotic expression vector pcDNA3.1 and transferred into INK4a/ARF locus-deficient leukemia cells HEL and K562. The changes in biological characteristics of the two p19ARF-transfected cells were observed. RESULTS: The growth of the p19ARF-transfected HEL cells was significantly inhibited compared with the vector-transfected cells; Cell cycle analysis showed that the expression of foreign p19ARF gene resulted in G1 phase cell cycle arrest and apoptosis cell death in some of HEL cells. However, p19ARF had no marked effects on the growth of K562 cells with p53 gene mutation and did not induce apoptosis in K562 cells. CONCLUSION: p19ARF suppressed the growth of leukemia cells by p53 -dependent pathway.
