Effects of thrombopoietin and thrombopoietinⅡ on human platelet activation
- VernacularTitle:血小板生成素及血小板生成素Ⅱ对人血小板活化的影响
- Author:
Tianhao LIU
;
Yunxian CHEN
;
Xueyun ZHONG
;
Liye ZHONG
;
Xubin LIN
;
Lin YAO
;
Hongyun ZHAO
;
Ruiming OU
;
Weiwei SU
;
Peili XU
- Publication Type:Journal Article
- Keywords:
Thrombopoietin;
Platelet activation;
Flow cytometry
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate effects of thrombopoietin(TPO) and TPOⅡ on human platelet activation in vitro. METHODS:Human platelets were incubated in the phosphate-buffered saline containing rhTPO or TPOⅡ at the concentration of 100 ?g/L for five minutes. In order to determine the rate of platelet activation. The CD62P and CD41 expressions on platelets were analysed by flow cytometry using fluorescence labelled monoclonal antibody to CD62P and CD41. RESULTS: The results demonstrated that expression of CD62P on platelets which were incubated with rhTPO or TPOⅡ didn't increase compared with that of contrast group. CONCLUSION: Both rhTPO and TPOⅡdidn't cause the disorder of platelet activation.
