Effects of intrathecal neostigmine on nitric oxide/cyclic guanosine 3',5'-monophosphate(NO/cGMP) signaling pathway of the spinal cord in a rat model of incisional pain
- VernacularTitle:在大鼠切口疼痛模型中鞘内新斯的明对脊髓NO/cGMP信号转导系统的影响
- Author:
Xingguo HU
;
Min ZHONG
;
Yinming ZENG
- Publication Type:Journal Article
- Keywords:
Pain;
Neostigmine;
Nitric oxide;
Cyclic GMP;
Injections,spinal
- From:
Chinese Journal of Anesthesiology
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of intrathecal neostigmine on nitric oxide synthase (NOS) activity, nitric oxide (NO) production and cyclic guanosine 3',5' monophosphate (cGMP) content of the spinal cord in a rat model of incisional pain Methods Male SD rats weighing 250 300g were anesthetized with intraperitoneal pentobarbital sodium 40 mg?kg -1 Intrathecal(IT) catheter was implanted and the tip of the cathter reached the lumbar region, according to the method of Yaksh Incision was made in the plantar aspect of the left hindpaw under 1 4% isoflurane anesthesia, according to the method of Brennan 64 male SD rats were were divided randomly into four groups GroupⅠ received IT 0 9% NaCl 20 ?l and 30 min later inhaled 1 4% isoflurane for 5 min but no incision was made Group Ⅱ received IT 0 9% NaCl 20 ?l 30 min before incision Group Ⅲ received IT neostigmine 10?g(10?l) 30 min after incision GroupⅣreceived IT neostigmine 10?g(10?l) 30 min before incision In group Ⅲ and Ⅳ 0 9%NaCl 10 ?l was flushed through IT catheter after IT neostigmine administration A cumulative pain score was utilized to assess pain behavior The animals were decapitated 2h after incision and the spinal cord was immediately removed and lumbar enlargement of the spinal cord was dissected on ice NOS activity, NO production and cGMP content were measured by spectrophotometry and radioimmunoassy Results Cumulative pain score in group Ⅲ and group Ⅳ was significantly lower than that in groupⅡ(P
