Effects of external counterpulsation on nitric oxide system in myocardial infarction canines
- VernacularTitle:体外反搏对心肌梗死犬一氧化氮系统的影响
- Author:
Xiaoxian QIAN
;
Zhensheng ZHENG
;
Weikang WU
;
Yanming CHEN
;
Shugang ZHANG
;
Guoquan GAO
;
Li LU
- Publication Type:Journal Article
- Keywords:
Myocardial infarction;
Nitric oxide;
Ligases;
Counterpulsation;
Dogs
- From:
Chinese Journal of Pathophysiology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of external counterpulsation (ECP) on nitric oxide (NO) and nitric oxide synthase (NOS) and the expression of NOS gene in myocardial infarction canines. METHODS: Nineteen healthy dogs were randomly divided into three groups ie. controls, ischemia group, ischemia and ECP group. Serum NO concentrations and myocardium NO levels and NOS specific activity were determined by modified nitrate reductase method. The protein synthesis of sub-type NOS including inducible NOS (iNOS) and endothelial NOS (eNOS) of myocardial tissue were also determined by immunohistochemical method. The constitutive NOS (cNOS) mRNA was measured via in situ hybridization. RESULTS: 120 and 180 minutes after the ligating of LAD, serum NO concentration in ECP groups were higher than those in ischemic groups (P
