Effects of oxidized high-density lipoprotein on membrane fluidity and the expression of lymphocyte function associated antigen-1 of monocyte
- VernacularTitle:氧化高密度脂蛋白对单核细胞膜脂流动性及淋巴细胞功能相关抗原-1表达的影响
- Author:
Houyuan HU
;
Yunzhen CHEN
- Publication Type:Journal Article
- Keywords:
Lipoproteins, HDL;
Monocytes;
Membrane fluidity;
Lymphocyte function-associated antigen-1
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effects of oxidized high-density lipoprotein (oxHDL) on membrane fluidity and the expression of lymphocyte function associated antigen(LFA-1) of monocyte. METHODS: The membrane fluidity of THP-1 cells was assayed by fluorescence anisotropy with DPH (1,6-dipheny-1,3,5-hexatriene), a fluorescent probe; The LFA-1 expression on THP-1 cells were assayed by flow cytometry with indirect immunofluorescence.RESULTS: The membrane fluidity of THP-1 cells was reduced by 45% and 52% respectively ( P
