Inhibitory effect and mechanism of Dsmosdumotin-C B ring derivative on MCF-7 cell proliferation
10.7501/j.issn.1674-6376.2017.02.007
- VernacularTitle:Desmosdumotin-CB环衍生物抑制MCF-7细胞增殖及其作用机制研究
- Author:
Hongju GUO
;
Lirong CHANG
;
Ning SHI
;
Jie ZHANG
;
Zhuo XIANG
;
Hai LIANG
;
Jiuhong WU
- Keywords:
dsmosdumotin-C;
derivative;
MCF-7;
apoptosis;
NF-κB
- From:
Drug Evaluation Research
2017;40(2):179-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effects of TPP,a desmosdumotin-C B ring para-fluoro modified derivative,on human breast cancer MCF-7 cell proliferation,and investigate the possible mechanisms.Methods MTT assay was used to measure the proliferation suppression of MCF-7 cells after treated with 1.0,2.5,5.0,10.0,and 20.0 μg/mL TPP for 48 h,and then the cell apoptosis rate and expression rate of NF-κB P65 positive cells were tested by flow cytometry after 20.0 μg/mL TPP treatment for 0,24,and 48 h.Results MTT assay showed that,after treatment for 48 h,1.0,2.5,5.0,10.0,and 20.0 μg/mL TPP all exhibited the inhibitory effects and showed a dose-dependent relationship.Flow cytometry results showed that 20.0 μg/mL TPP induced cell apoptosis after treatment for 24 and 48 h.TPP (20.0 μg/mL) significantly reduced the rate ofNF-κB P65-positive cells in MCF-7 cells after treatment for 48 h.Conclusion TPP could inhibit the proliferation of MCF-7 cells,which may be induced by cell apoptosis.Down-regulation of NF-κB is possible to be related with apoptosis.
