Axoplasmic Transport of Herpes Simplex Virus Co-Cultured with Ciliary Nerve.
- Author:
Suk Won KANG
1
;
Myung Kyoo KO
Author Information
1. Department of Ophthalmology, Hanyang University College of Medicine, Seoul, Korea. fovea@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Axonal transport;
Ciliary nerve;
Culture;
Herpes simplex virus;
Ultrastructure
- MeSH:
Axonal Transport*;
Axons;
Capsid;
Cells, Cultured;
Coculture Techniques;
Cytoplasm;
Herpes Simplex*;
Herpesvirus 1, Human;
Inclusion Bodies;
Microscopy, Electron, Transmission;
Nerve Growth Factor;
Simplexvirus*
- From:Journal of the Korean Ophthalmological Society
2005;46(9):1575-1581
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the replication of HSV within cultured cell and axonal transport of HSV within the axon of the ciliary nerve following the injection of HSV into a cultured ciliary nerve. METHODS: The explant of the ciliary nerve was cultured with a medium containing nerve growth factor for 30 days when the suspension of HSV-1 (Kos strain) was introduced into the culture dish to co-culture with the ciliary nerve. The ciliary nerve was examined with transmission electron microscopy 30 days after culture and 6 days after co-culture with HSV. RESULTS: The ultrastructure of the explant of the ciliary nerve co-cultured with HSV showed that the viral capsid acquired a viral envelope and viral core, and a capsid and inclusion body within the nucleus. The enveloped virus was scattered within the vesicles of the cytoplasm. The virus-like particles were identified at the axonal fibers. CONCLUSIONS: The co-culture of the explant of the ciliary nerve and HSV showed the replicative process of the HSV within the cultured cell. The virus-like particles within the axon showed the evidence axonal transport of the virus under culture conditions.
