Effects of Drought Stress on Activity of Key Enzyme in Saikosaponin Biosynthesis Passway and Saponins Content in Bupleurum chinense
10.3969/j.issn.1005-5304.2017.05.017
- VernacularTitle:干旱胁迫对柴胡中柴胡皂苷合成途径关键酶活性及柴胡皂苷含量的影响
- Author:
Xiaowei HAN
;
Hong FENG
;
Jianming FENG
;
Yuping YAN
;
Dan ZHANG
;
Kaiyan ZHENG
;
Yuguang ZHENG
- Keywords:
drought stress;
Bupleurum chinense;
activity of enzyme;
saikosaponin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(5):71-75
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the effects of drought stress on activity of key enzyme in saikosaponin biosynthesis and saponins content in Bupleurum chinense; To reveal response of Bupleurum chinense to drought stress from protein level. Methods Bupleurum chinense was cultivated in potted water control experiment with 70%–80%, 60%–70%, 40%–50% and 20%–30% saturated soil water contents. The enzyme activities of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase (HMGR), isoprenyl-based coke (IPPI), farnesyl pyrophosphate synthase (FPS) and β-carotenoid synthase (β-AS) of six months and one year old Bupleurum chinense were measured. The contents of saikosaponins a and d in samples of different soil water contents were determined by HPLC. Results When the saturated moisture was 40%–50%, the enzymatic activities of HMGR, IPPI, FPS and β-AS were the highest, followed by saturated moisture 60%–70%、70%–80% and 20%–30%. The trend of saikosaponin was similar to that of the activity of key enzyme. Conclusion 40%–50% soil saturated water content can significantly increase the activity of the key enzymes in the saikosaponin synthesis pathway. The saponin content and enzyme activity show a significant positive correlation, indicating that under drought stress, Bupleurum chinense regulates the synthesis of saikosaponin by regulating the key enzyme activity of saikosaponin synthesis pathway to respond drought stress.
