Mutations in C region of hepatitis B virus may contribute to occult hepatitis B virus infection
10.3969/j.issn.1006-5725.2017.04.008
- VernacularTitle:乙型肝炎病毒核心蛋白区突变与隐匿性乙型肝炎病毒感染的相关性
- Author:
Jingna CHEN
;
Tingting LI
;
Zhen WANG
;
Ying LIU
;
Wenjing WANG
;
Chengyao LI
;
Linhai LI
- Keywords:
Occult hepatitis B infection;
HBcAg;
HBsAg;
HBeAg;
HBV-RNA
- From:
The Journal of Practical Medicine
2017;33(4):533-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of mutations in C region that may contribute to occult hepatitis B virus infection.Methods C genes were amplified from two OBI blood donor samples respectively.Plasmids with mutations in C region of hepatitis B virus were constructed by overlapping PCR.HBsAg and HBeAg in Huh7 cells and in the serum of Balb/c mice were detected by CMLA.HBcAg in liver tissue was detected by immunohistochemistry,while HBV-RNA was tested by RT-PCR.Results Mutations in C region significantly reduced the expression level of HBeAg and HBcAg,but had no significant effect on HBsAg and HBV-RNA.Conclusion The mutations in C region affect the expression level of HBeAg and HBcAg,which may play an important role in the occurrence of OBI.
