Activation of small conductance Ca2+ activated K+ channelin spinal cord could inhibit morphine-induced hyperalgesia in mice
10.3969/j.issn.1001-1978.2017.04.019
- VernacularTitle:激活脊髓小电导钙离子激活钾通道可抑制小鼠吗啡痛觉过敏
- Author:
Junsheng ZHU
;
Gongliang ZHANG
;
Lei DU
;
Ningning JI
;
Siting HUANG
;
Yongmei ZHANG
;
Rong HUA
- Keywords:
SK channels;
1-EBIO;
morphine;
hyperalgesia;
spinal;
mice
- From:
Chinese Pharmacological Bulletin
2017;33(4):547-551
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the effect of activated SK channels(small conductance Ca2+-activated K+ channels) on morphine-induced hyperalgesia in the spinal cord in mice.Methods Adult C57BL6/N male mice were chosen to establish the model of morphine-hyperalgesia.The changes of tail withdrawal latency(TWL), mechanical withdrawal threshold(MWT) and the threshold of visceral pain were observed after intrathecal 1-EBIO, the agonist of SK channels.Results Compared with the control group, TWL, MWT and the threshold of visceral pain were decreased after morphine injection.After intrathecal 1-EBIO, the TWL, MWT and visceral pain threshold were increased.The level of spinal membrane SK2 expression in morphine-treated mice was decreased compared with that of control group.After intrathecal 1-EBIO, the level of spinal membrane SK2 expression was increased.Conclusion SK channels in the spinal cord are involved in morphine-induced hyperalgesia in mice.
