MRTF-A mediates FN and ICAM-1expressions by NF-κB pathway in AGEs-induced GMCs.
10.3969/j.issn.1001-1978.2017.04.024
- VernacularTitle:MRTF-A通过NF-κB参与AGEs诱导肾小球系膜细胞中FN、ICAM-1的表达
- Author:
Qiuhong CHEN
;
Zhiquan CHEN
;
Jiani HUANG
;
Heqing HUANG
- Keywords:
diabetic nephropathy;
glomerular mesangial cells;
AGEs;
MRTF-A;
p65;
FN;
ICAM-1
- From:
Chinese Pharmacological Bulletin
2017;33(4):572-577
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the expression of MRTF-A in rat glomerular mesangial cells(GMCs) induced by advanced glycation end products(AGEs) and its effect on ICAM-1 and FN;to explore whether MRTF-A is involved in the process of diabetic nephropathy by affecting NF-κB pathway.Methods Under the condition of AGEs, CCG-1423 and anti-MRTF-A small interfering RNA were used to knock down MRTF-A and MRTF-A plasmid was used to activatt MRTF-A, The expression level of MRTF-A, ICAM-1, FN and p65 in nucleus were detected by Western blot.Results The protein expressions of MRTF-A was increased in AGEs-induced GMCs.The expressions of FN and ICAM-1 and p65 in nucleus were downregulated by knocking down MRTF-A.However, the expressions of FN, ICAM-1 and p65 in nucleus were upregulated by overexpressing MRTF-A.Conclusions AGEs can upregulate the expression of MRTF-A in GMCs, and MRTF-A mediates the protein expressions of FN and ICAM-1 by affecting NF-κB signaling pathway in AGEs-induced GMCs.
