Role of S3I-201 in tubular interstitial lesion in lupus nephritis
10.13315/j.cnki.cjcep.2016.12.009
- VernacularTitle:S3I-201在狼疮鼠肾小管间质病变中的作用
- Author:
Yunxia DU
;
Wei ZHANG
;
Hongbo LI
;
Xiaojuan FENG
;
Qinhua WANG
;
Lingling XING
;
Hui WANG
;
Qingjuan LIU
- Keywords:
lupus nephritis;
tubular interstitial leison;
S3I-201;
STAT3
- From:
Chinese Journal of Clinical and Experimental Pathology
2016;32(12):1357-1360
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the role of S3I-201 on tubular interstitial lesion in lupus nephritis.Methods MRt/MpJ mice were designated as the control group.MRL/lpr nice were randomly divided into LN group,S3I-201 group and DMSO group.The serum and 24 h-urine were collected to detect the serum creatinine,blood urea nitrogen and urine protein.Immunohistochemistry was used to detect the expression of FN.Western blotting analysis was used to determine the expression of E-cadherin,α-SMA,MCP-1,ICAM1,STAT3 and p-STAT3.Results Compared with the expression level in control group,the protein level of α-SMA,MCP-1,ICAM1 and FN were increased in renal tissue of MRL/lpr mice,while the expression of E-cadherin was markedly decreased.And the STAT3 was activated in renal tissue of MRL/lpr mice.The administration of S3I-201 could inhibite the activation of STAT3 and ameliorate the expression of E-cadherin,α-SMA,MCP-1,ICAM-1 and FN.Conclusion S3I-201 can relieve the tubular interstitial leison,which maybe concerned with the phosphorylation of STAT3.
