Preliminary Study on Jianyi Recipe in Improving Islet Cell Function in Diabetic Rats Through Regulation of Glucagon-like Peptide-1 Expression
10.13359/j.cnki.gzxbtcm.2017.02.016
- VernacularTitle:健胰方通过调控胰高血糖素样肽-1表达改善糖尿病大鼠胰岛细胞功能的研究
- Author:
Xuerong YANG
;
Zhenhua ZHANG
;
Jie XU
;
Xin JIN
;
Junyan LI
;
Feng TAO
;
Hao LU
- Keywords:
Jianyi Recipe;
glucagon-like peptide-1;
diabetes mellitus/TCD therapy;
function of islet cells;
disease models,animal;
rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2017;34(2):213-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possible mechanism of Jianyi Recipe for improving the function of islet cells from the aspects of synthesis,secretion and inactivation of glucagon-like peptide-1 (GLP-1).Methods The diabetic rat model was established by feeding with high-lipid food combined with injection of streptozotocin (STZ).The rats were randomly divided into model group,Jianyi Recipe group,and normal group.The treatment for the rats lasted for 4 weeks.The blood glucose level was detected by the rapid blood glucose meter.The plasma levels of GLP-1 and insulin were detected by Luminex liquid phase protein chip technology.Pancreatic duodenal homeobox-1 (PDX-1) mRNA expression level was detected by quantitative real-time fluorescence polymerase chain reaction (PCR).The level of GLP-1 in ileum L cells was detected by immunohistochemistry,and dipeptidyl peptidase Ⅳ (DPP-Ⅳ)level was detected by enzyme-linked immunosorbent assay.Results Jianyi Recipe could decrease the levels of fasting blood glucose and postprandial glucose (P ＜ 0.05),promote the secretion of insulin (P ＜ 0.05),and increase PDX-1 mRNA expression level in the pancreas of the diabetic rats.Compared with the model group,plasma GLP-1 level,and ileal GLP-1 positive expression area and integrated optical density were increased (P ＜ 0.05) in Jianyi Recipe group,while the differences of serum DPP-Ⅳ levels were insignificant between the two groups (P＞ 0.05).Conclusion Jianyi Recipe maybe regulate the synthesis and secretion of GLP-1 to promote PDX-1 gene expression and insulin secretion,so as to reduce blood glucose in diabetic rats.
