Analgesic effect of oxysophoridine and its effect on brain GAT-1 in mice
10.3969/j.issn.1001-1978.2017.03.023
- VernacularTitle:氧化槐定碱对福尔马林致痛小鼠大脑GAT-1表达的影响
- Author:
Jinxian GAO
;
Xiujuan ZHANG
;
Jianqiang YU
;
Yuanxu JIANG
- Keywords:
oxysophoridine;
formalin;
analgesic;
GAT-1;
GABA;
brain
- From:
Chinese Pharmacological Bulletin
2017;33(3):407-411
- CountryChina
- Language:Chinese
-
Abstract:
Aim Tostudytheanalgesiceffectofoxyso-phoridine (OSR)on GABA transporter-1 (GAT-1 )mR-NA expression and its influence on GAT-1 expression inmice.Methods Formalintestwasusedtodetectthe analgesic effect of OSR(iv).Immunohistochemis-try was taken to inspect the expression of GAT-1 in cerebral cortex and thalamus in mouse brain. The quantitative real-time PCR method was used to inspect the influence of OSR on GAT-1 mRNA expression of braininmice.Results OSR(500,250,125mg· kg-1 ,iv ) could significantly increase the foot-licking latency.OSR(500 mg·kg-1,ip)could significantly decrease the number of GAT-1 immuopositive cells incerebral cortex and thalamus in mouse brain,and re-duce GAT-1 mRNA expression in brain(P<0. 01,P<0.05)intheformalintest.Conclusion OSRhasa significant analgesic effect,and its analgesic mecha-nism is related to the GAT-1 expression in mouse brain.
