Expression and Diagnostic Values of lncRNA-Malat-1, p21 and GAS5 in Colorectal Cancer Tissue
10.3870/j.issn.1672-0741.2017.01.003
- VernacularTitle:长链非编码核糖核酸Malat-1、p21和GAS5在大肠癌组织中的表达及其诊断价值
- Author:
Zhi TIAN
;
Lianghua LIU
;
Jiangtao LIAO
- Keywords:
lncRNA;
Malat-1;
p21;
GAS5;
colorectal cancer
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2017;46(1):10-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression and diagnostic values of long non-coding RNA(IncRNA)metastasis-associated lung adenocarcinoma transcript 1 (Malat-1),p21 and growth arrest-specific 5 (GAS5)in colorectal cancer tissue.Methods Coloreetal cancer of early stage and advanced stage(including paracareinoma tissue),precancerous lesion,benign tumor and noncancer lesion groups were collected,33 cases in each group.The levels of Malat-1,p21 and GAS5 in colorectal tissue of the 6 groups were detected by reverse transcription-polymerase chain reaction(RT-PCR).Serum carcinoembryonic antigen(CEA)and carbohydrate antigen19-9(CA19-9)were detected by ELISA.Results RT-PCR showed the levels of Malat-1,p21 and GAS5 were significantly higher in colorectal cancer of early and advanced stage and precancerous lesion than in the paracarcinoma lesion,benign tumor and non-cancer lesion(P＜0.05).Malat-1,p21 and GAS5 were the highest in the advanced stage group(P＜0.05).The levels of CEA and CA19-9 in non-cancer lesion were the least(P＜0.05).The AUC of ROC analysis operator characteristic curve(ROC)of Malat-1,p21 and GAS5 was comparable(P＞0.05).Pathological changes showed the pathological changes in colorectal cancer of early stage,advanced stage and precancerous lesion were more significant than those in the paracarcinoma lesion,benign tumor and non-cancer lesion (P＜ 0.05),which were the most significant in the advanced stage group.Conclusion The levels of Malat-1,p21 and GAS5.in colorectal cancer tissues could be good indicators for colorectal cancer diagnosis.
