Effect of H2 S system on space learning and memory ability in PTSD model rat and on the frequency of spontaneous discharges of hippocampus neurons
10.3969/j.issn.1001-1978.2017.02.013
- VernacularTitle:硫化氢对创伤后应激障碍大鼠空间学习记忆能力及海马神经元放电的影响
- Author:
Hongxia LIU
;
Lamei YU
;
Jin HUANG
;
Lixia ZHANG
;
Zhen YUE
;
Qingzhong LI
- Keywords:
H2 S;
CBS;
NaHS;
posttraumatic stress dis-order;
hippocampus;
in vivo extracellular single unit re-cordings
- From:
Chinese Pharmacological Bulletin
2017;33(2):206-211
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the behavior and CBS /H2 S levels of hippocampus in post traumatic stress dis-order (PTSD)rats and study the effect of exogenous H2 S on PTSD rats.Methods Single prolonged stress paradigm was adopted to prepare PTSD animal model. Morris water maze test was adopted to test space learn-ing and memory ability.CBS /H2 S content in hippo-campus tissue sample was measured using Western blot and methylene blue method.In vivo extracellular single unit recording was used to examine the frequency of spontaneous discharges of hippocampus neurons.Re-sults ① Escape latency increased and quadrant time (%)and platform crossing times decreased in Morris water maze test of PTSD group compared with normal group(P <0.01 ).CBS /H2 S level in hippocampus tis-sue of PTSD group also decreased compared with nor-mal group (P <0.01 ,P <0.05 ).② Escape latency decreased and quadrant time(%)and platform cross-ing times increased in Morris water maze test of NaHS+PTSD group compared with PTSD group(P <0.01 ).③ L-cysteine increased the frequency of spontaneous discharges of hippocampus neurons(P < 0.01 ).Con-clusions Learning and memory ability decrease in PTSD model rats owing to the inhibition of CBS /H2 S content in hippocampus tissue.The mechanism of be-havior improvement of H2 S on PTSD model rat is possi-bly related to the excitation of H2 S on frequency of spontaneous discharges of hippocampus neurons.
