Study on the Distribution of Germacrone from Zedoary Turmeric Oil in Tissues of Mice
10.6039/j.issn.1001-0408.2017.04.22
- VernacularTitle:莪术油中吉马酮在小鼠组织内的分布研究
- Author:
Huikao ZHANG
;
Dongdong WANG
;
Cheng SUN
;
Xiaomin LYU
;
Rong HU
- Keywords:
Zedoary turmeric oil;
Tissue distribution;
Germacrone;
HPLC;
Fluorescence observation
- From:
China Pharmacy
2017;28(4):512-514
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the distribution characteristics of germacrone from zedoary turmeric oil (ZTO) in each tis-sue of mice,and to provide reference for further application of zedoary turmeric oil. METHODS:30 KM mice were given zedoary turmeric oil 0.5 mL;6 mice were randomly selected 1,2,4,8,12 h after medication,respectively. The contents of germacrone in heart,liver,spleen,lung and kidney tissues were determined by HPLC. 15 KM mice were selected,medication and sampling method were same as above;3 mice were collected at each time point respectively. The fluorescence intensity of germacrone in above sections were observed by fluorescence. The same number of mice were selected as control in 2 trials. RESULTS:The con-centration of germacrone in each tissue 1-4 h increased gradually as time and reached the peak value at 4 h. The contents of ger-macrone in liver and spleen were significantly higher than in heart and lung. The concentrations of germacrone in each tissue were ranked as liver>spleen>kidney>heart>lung. The results of fluorescence intensity observation was same as above results. CON-CLUSIONS:Results of 2 methods show same distribution characteristics of germacrone in mice tissues,and indicate that ger-macrone is distributed more in liver,spleen and kidney tissues and less in heart and lung.
