Study on Isolation and Structure Characteristics of Immunoactive Polysaccharide RTPS-Ⅰfrom Trichosan-this Radix
- VernacularTitle:天花粉免疫活性多糖RTPS-Ⅰ的分离及性质结构研究
- Author:
Jiehong TU
;
Jia HUANG
;
Yinghua FU
;
Linqin XU
- Keywords:
Trichosanthis Radix;
Polysaccharide;
Isolation;
Physicochemical properties;
Structure analysis
- From:
China Pharmacist
2017;20(2):270-273
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To separate and purify the immunoactive polysaccharide RTPS- I from Trichosanthis Radix and study the physicochemical properties and preliminary structure. Methods:The crude polysaccharides were extracted by water, and then precipita-ted by ethanol. After the deproteinization with trichloroacetic acid, the polysaccharide RTPS-I was separated and purified by using DE-AE-Cellulose, DEAE-Sepharose FF and Sephadex G-200 column chromatography. The analysis of properties and structure was per-formed by using GC, HPGPC, IR and NMR. Results:RTPS-I was a white flocculent solid after vacuum freeze-drying, and soluble in water. It contained three elements (carbon, hydrogen and oxygen) with the specific rotation [α]25D (H2O) of +164. 44 and the relative molecular mass of 17555. The glycosyl in RTPS- I was composed of glucose without uronic acid. The sugar ring was pyranoid ring, and each monosaccharide was linked by α-glycosidic bond. Conclusion: A homogeneous immunoactive polysaccharide RTPS- I from Trichosanthis Radix is isolated for the first time.
