Role of TNF-αin Anterior Cingulate Cortex in Neuropathic Pain Induced by Sciatic Nerve Injury
- VernacularTitle:前扣带回内肿瘤坏死因子α过表达在神经病理性疼痛中的作用
- Author:
Shaokun WANG
;
Shaoxia CHEN
;
Peiwen YAO
;
Zicheng SUN
;
Xiaodong NA
;
Ying ZANG
- Keywords:
tumor necrosis factor-alpha;
anterior cingulate cortex;
neuropathic pain
- From:
Journal of Sun Yat-sen University(Medical Sciences)
2017;38(1):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective]To discuss the effect of sciatic nerve injury on the expressions of tumor necrosis factor-alpha(TNF-α), interleukin-1β(IL-1β)and interleukin-10(IL-10)in anterior cingulate cortex(ACC),and further to explain their roles resided in the development of neuropathic pain.[Method]With use of the methods of behavioral test,western blot and immunohistochemistry, we examine the effects of spared sciatic nerve injury(SNI)on the expressions of TNF-α,IL-1β,and IL-10 in ACC,and observe the effect of the neutralizing antibody of TNF-α,IL-1β on the rat mechanical allodynia.[Result]In present experiment ,SNI increased the protein levels of TNF-α,IL-10,but not IL-1β in ACC. Increased TNF-α-IR and IL-10-IR in ACC is located in neurons ,but not astrocytes and microglia at 7 d following L5-VRT. Pre-treatment with anti-TNFα antibody but not anti-IL-1βantibody into ACC significantly increased the rat paw withdrawal threshold to von Frey hairs.[Conclusion]These data suggested that the increased TNF-αin ACC neurons might be responsible for the development of neuropathic pain.
