Involvement of p38-p53 signal pathway in neuroprotective effects of DGMI on SH-SY5 Y cells damaged by oxygen-glucose deprivation
10.3969/j.issn.1001-1978.2016.12.013
- VernacularTitle:银杏二萜内酯葡胺注射液通过抑制p38／p53通路保护氧糖剥夺SY5 Y细胞的机制
- Author:
Xueke LI
;
Qiu LIU
;
Zhiliang XU
;
Jun ZHOU
;
Liang CAO
;
Gang DING
;
Zhenzhong WANG
;
Wei XIAO
- Keywords:
DGMI;
oxygen-glucose deprivation;
apop-tosis;
p38;
p53;
Bax/Bcl-2
- From:
Chinese Pharmacological Bulletin
2016;32(12):1699-1704
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effects of Diterpene Ginkgolides Meglumine Injection(DGMI)on SY5 Y cells damaged by oxygen-glucose deprivation and its functional mechanisms.Methods After 4 h of OGD,the cells were treated with 25 mg·L-1 drugs for 1 h.Subsequently,cell viabilities were measured by cell counting kit-8(CCK-8 kit)and cell apoptosis was measured by flow cytometric analysis.Furthermore, the mitochondrial membrane potential was detected by rhodamine123 staining.The levels of phospho-p38, phospho-p53,Bcl-2,Bax and cleaved caspase-9/3 were evaluated by western blot.Results DGMI signif-icantly increased the cell viabilities of SY5 Y cells dam-aged by OGD,and reduced OGD-elicited dissipation of mitochondrial membrane potential and cell apoptosis. Furthermore,DGMI also reduced p-p38,p-p53,Bax/Bcl-2 ratio,cleaved caspase-9 and cleaved caspase-3. Conclusion DGMI shows good neuroprotective effects on SY5 Y cells after oxygen-glucose deprivation.The underlying mechanisms may be associated with the sup-pression of p38/p53/Bcl-2 /caspase-9/caspase-3 sig-naling pathway.
