Effect of chlorogenic acid,caffeic acid,and ferulic acid on inhibition of inflammatory response of HMECs induced by activated complement alternative pathway
10.3969/j.issn.1001-1978.2016.12.017
- VernacularTitle:绿原酸、咖啡酸和阿魏酸对补体旁路激活致内皮细胞炎症相关分子表达的干预
- Author:
Ying ZHOU
;
Min LI
;
Qianyun SUN
- Keywords:
complement;
activation of the complement alternative pathway;
microvascular endothelial cells;
inflammation;
chlorogenic acid;
caffeic acid;
ferulic acid
- From:
Chinese Pharmacological Bulletin
2016;32(12):1723-1728
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of chlorogen-ic acid,caffeic acid,and ferulic acid on expression of molecules related with inflammatory response of HMECs induced by activated complement alternative pathway.Methods CVF was used to activate the al-ternative pathway of serum complement.After exposure of HMECs to activate complement for various times, supernatant of cell culture was removed and assayed for content of ICAM-1,IL-6,IL-8,t-PA,and PAI-1 u-sing ELISA kits.The expression of the above mole-cules induced by activated complement was measured after HMECs were pre-treated with 50,100,250 μmol ·L-1 of CGA,CA,and FA.Results After HMECs were exposed to the product of the activated comple-ment alternative pathway,the expression of ICAM-1 , IL-6,IL-8,t-PA,and PAI-1 was up-regulated.The expression of ICAM-1,IL-6,IL-8,t-PA,and PAI-1 was down-regulated by various concentrations of CGA, CA,and FA.ICAM-1 and IL-8 were inhibited most significantly in all molecules mentioned above.CA ex-hibited the best intervention effect,followed by FA. Conclusion Certain concentration of CGA,CA,and FA can inhibit the expression of ICAM-1,IL-6,IL-8, t-PA,and PAI-1 in HMECs induced by the activation of the alternative complement pathway,indicating that CGA,CA,and FA can inhibit inflammatory response of HMECs.
