Expression of miRNA-22 in ovarian cancer and effect of miRNA-22 over-expression on SKOV-3 ovarian cancer cell proliferation, migration and in-vasion
10.3969/j.issn.1000-4718.2016.12.021
- VernacularTitle:miRNA-22在卵巢癌组织的表达及其对卵巢癌细胞增殖、迁移与侵袭的影响
- Author:
Feng LI
;
Li YAO
;
Xihong ZHANG
;
Yuhong XIA
;
Jie CHENG
;
Xueling LOU
;
Qiuhui JIANG
- Keywords:
miRNA-22;
Ovarian cancer;
Cell proliferation;
Cell migration;
Cell invasion
- From:
Chinese Journal of Pathophysiology
2016;32(12):2251-2255
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22 over-expression on the proliferation, migration and invasion in SKOV-3 cells.METHODS: The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by qPCR .miRNA-22 was over-expressed by trans-fection of miRNA-22 mimic.The cell viability was examined by CCK-8 assay.The cell migration was measured by wound healing test .The cell invasion was analyzed by Transwell assay .The protein expression levels of VEGF and P 53 were deter-mined by Western blot .RESULTS: Compared with the normal ovarian tissue , the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues .After transfection with miRNA-22 mimic, the expression level of miRNA-22 in the SKOV-3 cells was significantly increased , while the cell viability , migration and invasion were obviously decreased .Moreover , the protein expression of VEGF and P 53 was dramatically inhibited after over-expression of miRNA-22.CONCLUSION:The decreased miRNA-22 expression may be correlated with the development of ovarian can-cer.Over-expression of miRNA-22 decreases the cell viability , migration and invasion by reducing the protein expression of VEGF and P53.
