Study on the carvacrol induced apoptosis in human non-small cell lung cancer cell line H1299
10.11958/20160597
- VernacularTitle:香芹酚诱导非小细胞肺癌1299细胞凋亡的实验研究
- Author:
Lei LUO
;
Yongkun WANG
;
Shikang LI
- Keywords:
carcinoma,non-small-cell lung;
apoptosis;
neoplasm invasiveness;
caspase 9;
matrix metalloproteinases 9;
tissue inhibitor of metalloproteinases;
Carvacrol;
human non-small cell lung cancer NCI-H1299 cells
- From:
Tianjin Medical Journal
2016;44(12):1418-1422
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study whether carvacrol can cause apoptosis in non-small cell lung cancer (NSCLC) cell line NCI-H1299, and explore its possible molecular mechanism. Methods NCI-H1299 cells were treated with different concentrations of carvacrol (20, 40, 60 and 80μmol/L) for 24 or 48 h. The viability of cells was evaluated by MTT assay, apoptosis was analyzed by flow cytometry (FCM) and the effect of carvacrol on metastasis of NCI-H 1299 was analyzed by Transwell assay. The expression level of caspase-9, MMP-9 and TIMP-1 were detected by quantitative realtime-PCR and Western blot assay. Results After treatment with carvacrol, the viability of NCI-H1299 cells was suppressed dramatically (P<0.05), without dose-dependent manner (P>0.05). After being incubated with carvacrol for 24 h, FCM analysis indicated that carvacrol effectively induced apoptosis in NCI-H1299 cells (P<0.05), without dose-dependent manner (P>0.05). The ability of invasion was decreased (40.67±3.63 vs. 76.00±5.78). Carvacrol inhibited the protein and mRNA expression levels of caspase-9, but increased the expression of MMP-9 and TIMP-1 (P<0.05). Conclusion Carvacrol can induce apoptosis of NCI-H1299 cells and inhibit their invasion, which may be associated with up-regulation of caspase-9 expression and down-regulation of MMP-9 expression.
