Effect of ClC-3 siRNA on cell cycle of HeLa cells
10.3969/j.issn.1000-4718.2017.02.011
- VernacularTitle:沉默 ClC-3氯通道基因对 HeLa 细胞周期分布的影响
- Author:
Dong YE
;
Degang XING
;
Zhi ZENG
;
Lixin CHEN
;
Liwei WANG
- Keywords:
ClC-3 chloride channels;
HeLa cells;
Cell cycle;
Cyclin-dependent kinase;
P21;
P27
- From:
Chinese Journal of Pathophysiology
2017;33(2):257-262
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the roles of ClC-3 chloride channels in the regulation of cell cycle and the re-lationship between ClC-3 chloride channels and the cell cycle regulators , such as cyclin D1, cyclin-dependent kinase (CDK)4, CDK6, P21 and P27 in the HeLa cells.METHODS:ClC-3 genes were silenced by the siRNA technique in the HeLa cells.The transfection efficiency of ClC-3 siRNA was detected by real-time PCR.The cell cycle distribution was ana-lyzed by the flow cytometry .The protein expression of ClC-3, P21, P27, CDK4, CDK6 and cyclin D1 was determined by Western blot .RESULTS:ClC-3 was knocked down by ClC-3 siRNA in the HeLa cells .Transfection of the cells with ClC-3 siRNA arrested the cells at G0/G1 phases, decreased the expression of cyclin D1, CDK4 and CDK6, and increased the expression of P21 and P27.CONCLUSION:ClC-3 plays an important role in the cell cycle of HeLa cells through the G 1-S transition point.ClC-3 may regulate the cell cycle progression by up-regulation of cyclin D1, CDK4 and CDK6 expression and/or by down-regulation of P21 and P27 expression.
