Characteristics of 99Tcm-MIBI hepatobiliary scintigraphy in biliary atresia model and its association with the expression of P-glycoprotein in intestinal tissues
10.3760/cma.j.issn.2095-2848.2016.06.012
- VernacularTitle:大鼠胆道闭锁模型99Tcm-MIBI肝胆显像特点及与肠道组织P-糖蛋白表达的相关性
- Author:
Yongshuai QI
;
Guiping LI
;
Li DU
;
Baodan HUANG
;
Quanshi WANG
;
Hubing WU
;
Xiaohua CHI
- Keywords:
Biliary atresia;
P-glycoprotein;
Radionuclide imaging;
Rats;
MIBI
- From:
Chinese Journal of Nuclear Medicine and Molecular Imaging
2016;36(6):533-537
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish rat models of extrahepatic biliary atresia,and to observe the characteristics of 99Tcm-MIBI hepatobiliary scintigraphy and evaluate its association with the expression Pglycoprotein (P-gp) in intestinal tissues.Methods A total of 12 SD rats were randomly divided into the normal control group (3 rats) and the group of common bile duct ligation (CBDL;9 rats).CBDL was used to establish the rat model of extrahepatic biliary atresia.99Tcm-MIBI hepatobiliary scintigraphy was performed at 2,3 and 4 weeks after ligation in the CBDL group and normal control group with continuous dynamic acquisition (3 min/frame) for 30 min and then delaying imaging at 30 min,1,2 and 3 h.After that,all rats were sacrificed,and the blood samples were taken out for the determination of serum ALT,AST,TBIL,DBIL,IBIL,ALP,γ-GT and TBA,and the tissues of duodenum,jejunum,ileum,colon and cecum were taken out for analyzing the expression level of P-gp by immunohistochemistry.Two-sample t test and one-way analysis of variance were used.Results Compared with the normal control group,the serum levels of ALT,AST,TBIL,DBIL,IBIL,ALP,γ-GT and TBA were significantly increasing at 2,3,4 weeks after ligation in CBDL group (t:-3.04 to-44.54,all P＜0.05).99Tcm-MIBI hepatobiliary imaging showed that there was radioactive accumulation in colon or cecum area,excluding the duodenum,jejunum and ileum area,at 3 h after intravenous injection of 99Tcm-MIBI in CBDL group.The results of immunohistochemistry showed that with the obstruction time prolonged,the expression levels of P-gp in duodenum,jejunum and ileum segments were gradually decreased (F=5.17,9.07,23.52;all P＜0.05),while the expression levels in the colon and cecum segments were not changed obviously (F=2.00,3.17;both P＞0.05).Conclusion 99Tcm-MIBI can be excreted through intestinal mucosa,and this process may be associated with P-gp expression.
