Expression and significance of NLRP3 inflammatory body in neonatal rats with hypoxic-ischemic brain damage
10.3760/cma.j.issn.1674-6554.2017.01.003
- VernacularTitle:NLRP3炎性小体在缺氧缺血新生大鼠脑组织中的表达及意义
- Author:
Xiaoguang LI
;
Molin WANG
;
Wenzhe LUO
;
Dezhi PANG
;
Jiabin SUN
;
Lihua ZHANG
- Keywords:
Inflammatory;
NLRP3;
Neonatal rat;
Cerebral ischemia and hypoxia
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of NLRP3 in different time point of HIBD neonatal rats and to search for critical time points and alleviate HIBD dysfunction.Methods 96 newborn rats of 7 days old were randomly divided into HIBD group(n=48) and Sham operation group(n=48).HIBD model was prepared by referring to Rice method.Brain tissue was taken after 6 h,24 h,72 h,7 d.Brain injury was detected by HE stain.The expression and distribution of NLRP3 and Caspase-1 were detected by immune fluorescence and Western blot,and IL-1β and IL-18 were detected by ELISA.Results HE staining and immunofluorescence showed that NLRP3 protein (HIBD group (0.63±0.07),Sham group(0.43±0.04)) was increased significantly since 6 h in HIBD group,and its downstream protein Caspase-1,IL-1β and IL-18 were successive activated.The results showed IL-1β (HIBD group(732.28± 108.42)pg/ml,Sham group(584.58± 36.35) pg/ml) was increased significantly since 6 h in HIBD group;Caspase-1 (HIBD group(0.67±0.09),Sham group(0.30±0.05)),IL-18 (HIBD group(683.84±31.83) pg/ml,Sham group(571.32±50.91) pg/ml) was increased significantly since 24 h in HIBD group(P＜0.05).Conclusion NLRP3 and its downstream inflammatory cytokines IL-1 β and IL-18 are up-regulated when HIBD occurs.The change of NLRP3protein expression in group HIBD is earlier than changes of neuron.NLRP3 signal may mediate and participate in the occurrence and development of HIBD.
