Content Determination of Baicalin in Yangyin Mixture by HPLC
10.6039/j.issn.1001-0408.2016.24.30
- VernacularTitle:HPLC法测定养阴合剂中黄芩苷的含量
- Author:
Keke CHE
;
Fan YANG
;
Kaiying DENG
- Publication Type:Journal Article
- Keywords:
Yangyin mixture;
Baicalin;
HPLC;
Determination
- From:
China Pharmacy
2016;27(24):3410-3412
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of baicalin in Yangyin mixture. METHODS:HPLC was performed on the column of SunFire C18 with mobile phase of methnol-water-phosphoric acid(43∶57∶0.2,V/V/V)at a flow rate of 1.0 ml/min,the detection wavelength was 280 nm, column temperature was 30 ℃,and the volume injection was 10 μl. RESULTS:The linear range of baicalin was 0.055 75-1.115 μg(r=0.999 9);RSDs of precision,stability and reproducibility test were lower than 2%;recovery was 95.39%-98.44%(RSD=1.19%,n=6). CONCLUSIONS:The method is simple,rapid and accurate,and suitable for the content determination of baicalin in Yangyin mixture.
