Application of Xpert MTB/RIF assay on rapid detection of MTB and rifampin-resistant
10.3969/j.issn.1673-4130.2016.18.024
- VernacularTitle:Xpert MTB／RIF在快速诊断肺结核及利福平耐药中的临床应用
- Author:
Hongjing ZHOU
;
Mingri GUO
;
Shuang FENG
;
Lixia ZHANG
- Publication Type:Journal Article
- Keywords:
Mycobacterium tuberculosis;
Xpert MTB/RIF;
drug-resistance
- From:
International Journal of Laboratory Medicine
2016;37(18):2568-2570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate clinical value of Xpert M TB/RIF assay on rapid detection of M TB and rifampin‐resistant . Methods 408 cases of tuberculosis were detected by acid‐fast stain ,Solid culture and Xpert MTB/RIF ,the results were compared . Results All the samples were detected by acid‐fast stain ,culture and Xpert MTB/RIF assay ,the positive rates were 25 .00% (102/408) ,42 .16% (172/408) and 58 .09% (237/408) ,respectively .The positive rates of Xpert MTB/RIF assay were higher than acid‐fast stain and culture method ,and the difference is statistically significant(P<0 .05) .There are 168 cases of specimen which were tested positively by both culture and Xpert M TB/RIF ,the RIF resistance rates from standard drug susceptibility testing and Xpert MTB/RIF assay respectively were 26 .19% (44/168) and 27 .38% (46/168) ,and had no statistical significance(P>0 .05) .Accord‐ing to the result of drug susceptibility with proportion method ,the sensitivity and specificity of Xpert MTB/RIF in detecting RIF resistance were 97 .73% (43/44) and 97 .58% (121/124) .Conclusion Xpert MTB /RIF can quickly and accurately detect MTB and RIF resistance ,it has important clinical significance .
