Content Determination of Glabridin in Clycyrrhizae Radix et Rhizoma from Different Regions by HPLC
10.6039/j.issn.1001-0408.2016.06.33
- VernacularTitle:HPLC法测定不同产地甘草中光甘草定的含量
- Author:
Xinhua HU
;
Wanshan CHEN
;
Jinsong YUAN
- Publication Type:Journal Article
- Keywords:
HPLC;
Clycyrrhizae Radix et Rhizoma;
Different regions;
Glabridin;
Content determination
- From:
China Pharmacy
2016;(6):824-826
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the content determination of glabridin in Clycyrrhizae Radix et Rhizoma from different regions. METHODS:HPLC was performed on the column of Inertsil ODS-SP with mobile phase of acetoni-trile-0.05%phosphoric acid(gradient elution)at a flow rate of 0.8 ml/min,detection wavelength was 280 nm,the column tempera-ture was 35 ℃,and the injection volume was 20 μl. RESULTS:The linear range of glabridin was 0.906-18.12 μg/ml(r=0.999 7), RSDs of precision,stability and reproducibility tests were lower than 3%;recovery was 98.73%-101.90%(RSD=1.25%,n=6). There were obvious differences among the glabridin contents in G. uralensis from different regions. CONCLUSIONS:The method is simple and accurate with high precision,and can be used for the content determination of glabridin in Clycyrrhizae Radix et Rhi-zoma.
