Expression profiles of CaMKIIδat different stages of osteoclast differenti-ation
10.3969/j.issn.1000-4718.2016.10.021
- VernacularTitle:CaMKIIδ在破骨细胞分化不同阶段表达规律的研究
- Author:
Dazhuang LU
;
Juanjuan LIU
;
Mengchun QI
;
Liming WEN
;
Ren LI
;
Hong SUN
- Publication Type:Journal Article
- Keywords:
Ca2+/calmodulin-dependent protein kinase II delta;
Osteoclasts;
Receptor activator of nuclear factor κB ligand;
Tartrate-resistant acid phosphatase
- From:
Chinese Journal of Pathophysiology
2016;32(10):1870-1874
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To study the expression profiles and the role of Ca 2+/calmodulin-dependent kinase II delta ( CaMKIIδ) during osteoclast differentiation .METHODS:Mouse RAW264.7 cells were induced by receptor activator of nuclear factor κB ligand ( RANKL) at 50μg/L for osteoclastogenesis .Tartrate-resistant acid phosphatase ( TRAP) staining and bone resorption lacunae examination were performed to verify osteoclast formation .The expression of CaMKIIδat mR-NA and protein levels was also determined by immunofluorescent cytochemistry , RT-qPCR and Western blot at days 0, 1, 3 and 5.RESULTS:TRAP positive multinuclear cells with bone resorption function were formed after 5 d of induction. The mRNA levels of CaMKIIδdetected by RT-qPCR were 1.028 ±0.041, 2.478 ±0.087, 10.524 ±1.284 and 42.914 ± 2.667 at days 0, 1, 3 and 5, respectively, while the protein levels of CaMKIIδ detected by Western blot were 0.762, 0.963, 1.802 and 3.136, respectively.The changes of protein level were also verified by immunofluorescence cytochemis -try, in which the fluorescence intensity increased in a time-dependent manner (P<0.05).CONCLUSION:The expres-sion of CaMKIIδincreases with the differentiation of osteoclasts .CaMKIIδmay play a key role in the osteoclastogenesis .
