Simultaneous Determination of Coumarin Compounds in Yifu Pill by HPLC
10.6039/j.issn.1001-0408.2016.30.40
- VernacularTitle:HPLC法同时测定益肤丸中香豆素类化合物的含量
- Author:
Xiaoling LI
;
Juanjuan DIAO
;
Xinxia LI
- Publication Type:Journal Article
- Keywords:
Yifu pill;
Osthole;
Isoimperatorin;
Columbianadin;
Content determination;
HPLC
- From:
China Pharmacy
2016;27(30):4299-4301
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of coumarin compounds in Yifu pill. METH-ODS:HPLC was performed on the column of Inertsil ODS-3 with mobile phase of acetonitrile-water(49∶51,V/V) at a flow rate was 1.0 ml/min,the detection wavelength was 320 nm,column temperature was 30 ℃,injection volume was 20 μl. RESULTS:The linear range was 5.16-206.4μg/ml for osthole (r=0.999 9),37.40-1 495.89μg/ml for isoimperatorin(r=0.999 9) and 9.95-318.34 μg/ml for columbianadin(r=0.999 9);RSDs of precision,stability and reproducibility tests were lower than 3.0%;recov-eries were 96.05%-103.19%(RSD=2.18%,n=9) for osthole,96.90%-103.09%(RSD=2.07%,n=9) for isoimperatorin, 95.50%-103.57%(RSD=2.15%,n=9)for columbianadin. CONCLUSIONS:The method is simple with good precision,stability and reproducibility,and can be used for the simultaneous determination of coumarin compounds in Yifu pill.
